载药磷酸钙人工骨的体外实验研究

VITRO EMPIRICAL STUDY ON DRUG-LOADED CALCIUM PHOSPHATE CEMENTS

评价载药自固化磷酸钙人工骨(CPC)的体外抑菌特点。将CPC粉末按5%的比例复合万古霉素、妥布霉素、头胞唑啉钠、环丙沙星,各取3粒载药CPC,分别置于接种有金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌的琼脂固体培养基中,测量抑菌圈直径,取无药的CPC12粒,观察其抑菌作用。结果环丙沙星在2天后仍不能很好固化,放弃进一步试验。载药CPC颗粒对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌均有明显的抑菌圈,载妥布霉素CPC对铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌抑菌作用分别达3、9、12周。载万古霉素CPC对大肠杆菌、铜绿假单胞菌的抑菌作用达6周,对金黄色葡萄球菌的抑菌作用达12周以上。载头孢唑啉钠CPC对大肠杆菌、铜绿假单胞菌的抑菌作用达3周,对金黄色葡萄球菌的抑菌作用为5周,明显短于妥布霉素、万古霉素。而对照组无抑菌作用。表明载药CPC在体外具有较强的、持续时间较长的抑菌活性,具有临床上应用于修复骨缺损和治疗骨髓炎的广阔前景。

To evaluate the bacteriostasis characteristics of drug-loaded calcium phosphate cements(CPC) in Vitro.CPC powder was respectively compounded in radio of 100:5 with Vancomycin,Tobramycin,Cefazolin and Ciprofloxacin.12 pills of drug-loaded CPC were selected to be put in the three different agar solid media,which were inoculated with staphylococcus aureus,escherichia coli and pseudomonas aeruginosa respectively,3 pills in each.The diameters of bacteriostasis area were measured.And 12 pills of inantherate CPC were chosen and observed their bacteriostatic action.The drug-loaded CPC with ciprofloxacin was abandoned for it s unsatisfactory solidification two days later.All of them had conspicuous bacteriostasis area on staphylococcus aureus,eschericliia coli and pseudomonas aeruginosa.The times of bacteriostatic action on drug-loaded CPC with tobramycin pills to pseudomonas aeruginosa,eschericliia coli and staphylococcus aureus were 3 weeks,9 weeks and 12 weeks respectively.Those of drug-loaded CPC with vancomycin to escheriehia coli and pseudomonas aeruginosa were 6 weeks,and above 12 weeks to staphylococcus aureus.Those of drug-loaded CPC with cefazolin sodium to eschericliia coli and pseudomonas aeruginosa were 3 weeks,5 weeks to staphy'ococcus aureus,and obviously shorter than tobramycin and vancomycin.But control group had no bacteriostatic action.It showed that drug-loaded CPC had strong action and long-time activity in bacteriostasis,and a good foreground on repairing bone defect and treatment of osteomyelitis,