氯霉素、利福平对肺炎支原体的抗菌活性分析

Analysis on Antibacterial Activity of Chloramphenicol and Rifampin to Mycoplasma Pneumoniae

目的探讨氯霉素、利福平对肺炎支原体(MP)的抗菌活性。方法对370例咽拭子标本进行MP分离培养,应用套式聚合酶链式反应(PCR)扩增MP种特异16SrRNA基因对临床分离株进行分子鉴定;通过药物敏感试验测定MP分离株的红霉素的最小抑菌质量浓度(MIC),应用套式扩增红霉素作用靶位23SrRNA基因,以鉴别敏感株和耐药株;应用药物敏感试验测定MP分离株的氯霉素、利福平的MIC。结果临床标本370例中分离MP50株。其中红霉素敏感株4株,耐药株46株。46株耐药株的红霉素作用靶位23SrRNA基因发生点突变,4株敏感株无点突变。所有MP分离株对氯霉素敏感,对利福平耐药。结论利福平对MP无效,氯霉素对MP红霉素敏感株及耐药株均有效。

Objective To explore the antibacterial activity of chloramphenico and fifampin to mycoplasma pneumoniae（MP）. Methods Three hundred and seventy throat swab specimens were cultured to isolate MP. Clinical isolated strains were identified by nested polymerase chain reaction（PCR） of MP species specific 16SrRNA gene. Minimal inhibitory concentration（MIC） of erythromyein was determined by antibiotic susceptibility test and 23SrRNA gene was amplified by nested PCR to identify macrolide resistant strains. MIC of rifampin and chloramphenicol were determined by antibiotic susceptibility test. Results Fifty clinical strains were isolated from 370 specimens. Of 50 strains,4 strains were suscepti- ble to erythromycin,while 46 strains were erythromycin resistant. Fclrty - six resistant strains bad a point mutation respectively in 23SrRNA gene, 4 susceptible strains had not point mutation. All isolated strains were susceptible to chloramphenicol and resistant to rifampin. Conclusion Rifampin is not effective to MP,and Chloramphenicol is effective to erythromycin susceptible and resistant MP strain.