CCK对犬Oddi括约肌电生理影响的实验研究

Effect of CCK on myoelectric motility of sphincter of Oddi in dogs

目的探讨不同剂量的胆囊收缩素（cholecystokinin,CCK）对犬Oddi括约肌（sphincter of oddi,SO）肌电活动的影响。方法8只成年家犬麻醉成功后,用Pull-through法将黏膜接触电极置于SO内,分别记录静脉注射CCK前、注射生理剂量的CCK（20ng/kg）及大剂量的CCK（100ng/kg）后SO的肌电活动,每次记录60~80min。结果静脉注射生理剂量的CCK后SO的慢波（slow wave of SO,SWSO）波幅降低、频率变慢,SO肌电簇（myoelectronic activity of SO,MASO）波幅也降低、频率明显变慢（P〈0.05）;大剂量给予CCK后SO的慢波和肌电簇波幅都明显增高、SO肌电簇频率增快（P〈0.05）。结论生理剂量的CCK对犬SO运动具有抑制作用,导致SO处于舒张状态;增大剂量的CCK对SO的作用为兴奋作用,表现为肌电活动增强。

Objective To explore the effect of different doses of cholecystokinin （CCK） on the myoelectric motility of sphincter of Oddi （SO） in dogs. Methods Eight adult mongrel dogs was anesthetized, the membrane contact electrode was inserted into the SO by Pull-through method. The myoelectric motilities of SO before injection CCK, after injection 20 ng/kg CCK and 100 ng/kg CCK were recorded,each dog recorded 60--80 min. Results After bolus injection physiological dose of CCK（20 ng/kg）, the slow wave amplitude and frequency of SO decreased, the myoelectronic activity amplitude and frequency of SO also decreased（P〉0. 05）. After bolus injection large dose of CCK（100 ng/kg）, the slow wave amplitude of SO increased, the myoelectronic activity amplitude and frequency of SO increased too（P〉0.05）. Conclusion Physiological dose of CCK has an inhibitory effect on the SO, which result in the relaxation of SO. Whereas the large dose of CCK has an stimulatory effect, which induced the enhanced myo- electic motility of SO.