ApoE 基因多态性与中国人非胰岛素依赖型糖尿病血脂水平的关系

RELATIONSHIP BETWEEN ApoE GENE POLYMORPHISM AND THE SERUM LIPID LEVELS IN CHINESE WITH NIDDM

为研究ＡｐｏＥ基因对中国人非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者血脂水平的影响，应用聚合酶链反应（ＰＣＲ）扩增出ＡｐｏＥ基因第４外显子内的２９２ｂｐ片段，继以限制性内切酶ＨｈａⅠ酶解消化，最后行８％聚丙烯酰胺凝胶电泳，根据电泳带型判断ＡｐｏＥ基因型。共检测了１１２例非胰岛素依赖型糖尿病患者的ＡｐｏＥ基因型。通过比较不同ＡｐｏＥ基因型与血脂水平的关系发现：ＡｐｏＥ基因多态性与ＴＧ，ＨＤＬ－Ｃ，ＡｐｏＡⅠ，ＡｐｏＢ及Ｌｐ（ａ）水平无相关性，而与ＴＣ（Ｐ＝０．００２９）及ＬＤＬ－Ｃ（Ｐ＝０．００２１）水平相关，携带ε４等位基因者具有较高的ＴＣ及ＬＤＬ－Ｃ水平，而携带ε２等位基因者具有较低的ＴＣ及ＬＤＬ－Ｃ水平。

To study the effects of Apo E gene on the serum lipid levels in Chinese with non insulin dependent diabetes mellitus(NIDDM), a 292 base pair DNA fragment in the fourth exon of ApoE gene was amplified by polymerase chain reaction (PCR), analyzed by digestion with restriction enzyme Hha Ⅰ, and followed by 8% polyacrylamide gel electrophoresis of the cleavage products, subsequently the Apo E genotype was determined in 112 NIDDM patients. Comparing the values of the serum lipids among NIDDM patients with different genotypes, we found no impact of the various Apo E genotypes on the serum levels of TG, HDL C, ApoA I, Apo B and Lp(a), but Apo E gene polymorphism was significantly associated with TC( P =0.0029) and LDL C( P =0.0021) in NIDDM patients. NIDDM individuals carrying ε4 allele were associated with increased concentrations of TC and LDL C, while those carrying ε2 allele were associated with decreased concentrations of TC and LDL C.