慢性非细菌性前列腺炎/慢性骨盆疼痛综合征患者外周血Th_1/Th_2淋巴细胞比值的免疫学研究

Detection of Peripheral Blood Th_1/Th_2 Cell Ratio in Patients with Chronic Abacterial Prostatitis/Chronic Pelvic Pain Syndrome

目的:探讨慢性非细菌性前列腺炎/慢性骨盆疼痛综合征(CAP/CPPS)患者外周血Th1/Th2细胞分布的变化情况,了解其在CAP/CPPS发病过程中的意义。方法:采用流式细胞学检测40例CAP/CPPS患者和20例健康体检者外周血CD3+CD8-T细胞内细胞因子干扰素γ(IFN-γ)和白细胞介素(IL-4)的表达。结果:与正常对照相比,Ⅲa型、Ⅲb型CAP/CPPS患者的Th1细胞数均升高,Th1/Th2比值升高,差异有显著性(P<0.05),Th2细胞数差异无显著性(P>0.05)。结论:CAP/CPPS患者Th1/Th2平衡失调,Th1型免疫反应模式占优势状态,提示Th1细胞在CAP/CPPS的疾病发生中可能起重要作用。

Objective To investigate the change of peripheral Th1/Th2 cells in patients with CAP/CPPS and evaluate its in the etiology of CAP/CPPS. Methods Interferon-γ （IFN-γ） and interleukin-4 （IL-4） secreted by CD3^＋ CD8^＋ T cells were detected by flow cytometry in 40 patients with CAP/CPPS and 20 healthy volunteers. Results Compared with control group ,peripheral Th1 cells were significantly increased in patients with Ⅲa and Ⅲb CAP/CPPS. The Th1/Th2 cells ratio was apparently creased （P 〈 0.05 ）, while Th2 cells was not different from that in the control （P 〉 0.05 ）. Conclusion There is a Th1/Th2 imbalance in CAP/CPPS patients with an increase of Th1 type cytokines, Th1 cell may play an importantrole in the pathogenesis of CAP/CPPS.