摘要
采用抗人凝血因子Ⅸ单克隆和多克隆两种抗体,建立了人凝血因子Ⅸ抗原(FⅨ∶Ag)酶联免疫测定法(ELISA)。标准曲线相关系数为0.993(P=0.001,n=5),测定范围(6.25~100)U/L,最低检测量1.5U/L;批内及批间变异系数分别为7.25%和4.73%,回收率99.7%;与人血浆中其它蛋白质未见交叉反应。测得20人份正常人血浆中FⅨ∶Ag为(1027±172)U/L,与FⅨ促凝活性(FⅨ∶C)测得值比较,差异无显著性(P>0.5)。重症乙型血友病人血浆FⅨ∶Ag测得值与临床诊断相符。
An enzyme linked immunosorbent assay(ELISA) for measuring human coagulation factor Ⅸ antigen (FⅨ∶Ag) has been established,using both monoclonal and polyclonal antibodies to FⅨ.The standard curve has a correlation coefficent r=0.993(n =5).This assay enabled the determination of FⅨ∶Ag in concentration from 6.25U/L to 100U/L.The lowest value of detection was 1.5U/L.Within run and between run coefficients of variation were 7.25% and 4.73% respectively.The recovery rate was 99.7%.There was no cross reaction with other proteins in human plasma.The mean concentrations of FⅨ∶Ag in plasma of 20 normal subjects was (1027±172)U/L.When the results were compared to those obtained by the determination of FⅨ coagulate activity,the difference between two groups wanst significant( P >0 5).The FⅨ∶Ag of the plasma in patient with severe hemophilia B were tallied with clinical diagnosis.
出处
《中国输血杂志》
CAS
CSCD
北大核心
1997年第1期3-5,共3页
Chinese Journal of Blood Transfusion
关键词
凝血因子IX
酶联免疫法
单克隆抗体
FⅨ
Enzyme linked Immunosorbent Assay
Monoclonal antibody.