80℃,72h干热处理FⅧ制剂中指示病毒灭活效果的考察

Observation of Effectiveness of Indicant Virus Inactivation in FⅧ Concentrate by Heat dry Treatment at 80℃ for 72 Hours

以水泡性口炎病毒（ＶＳＶ）、Ｓｉｎｄｂｉｓ和ＰｏｌｉｏＩ型疫苗病毒为指示病毒，考察８０℃，７２ｈ干热处理对ＦⅧ制剂中的病毒灭活效果。结果表明，该法对加入制品中的ＶＳＶ、Ｓｉｎｄｂｉｓ和ＰｏｌｉｏＩ型疫苗病毒的灭活能力分别为８．３、９．１和７．７ｌｇＴＣＩＤ５０／ｍｌ；证实８０℃，７２ｈ干热处理，对模拟脂包膜和非包膜病毒的指示病毒ＶＳＶ、Ｓｉｎｄｂｉｓ和ＰｏｌｉｏＩ型疫苗病毒，均可起到有效的灭活作用。

The effectiveness of virus inactivation in FⅧ concentrate by heat dry treatment at 80℃ for 72 hours was observed,taking VSV,Sindbis and Polio Ⅰ type vaccina virus as indicant virus.The results showed that the capability of heat dry method to inactivate VSV,Sindbis and Polio Ⅰ type vaccina virus in FⅧ concentrate was 8.3,9.1 and 7.7 lg TCID 50 /ml,respectively.It was confirmed that the heat dry treatment at 80℃ for 72 hours is an effective inactivation method for lipid enveloped and non lipid enveloped virus such as VSV,Sindbis and Polio Ⅰ type vaccina virus.