水稻瘤矮病毒基因组第6片段(S6)序列测定与分析

Sequencing and Analysis of Rice Gall Dwarf Virus Genome Segment 6

【目的】了解水稻瘤矮病毒(RGDV)基因组结构与功能。【方法】根据已报道的RGDV基因组各片段末端序列及其反向重复特性,采用4轮RT-PCR的方法扩增RGDV基因组第6片段(S6),用生物学软件进行序列分析。【结果】得到了该病毒S6的全序列。S6全长1651bp,G+C含量39.13%,包含一个长的ORF,起始于第25位,终止于第1497位,编码490个氨基酸,分子量为53.3kD。【结论】该蛋白与伤瘤病毒(WTV)的P7的相似性为30.0%,与水稻矮缩病毒(RDV)的P7的相似性为31.7%。这是RGDVS6全序列的首次报道。

[Objective] In order to understand the whole genome organization and its functions of Rice gall dwarf virus （RGDV）, we obtained the complete sequence of RGDV genome segment 6. [Method] Four rounds of RT-PCR were performed according to the property of segment-specific reverted repeat termini sequences reported. Sequences were analyzed by bio-software. [Result] The full-length cDNA of S6 was 1 651 bp with 39.13% G＋C content, containing a single open reading frame （ORF） that extends l 473 nucleotides from nucleotide 25. The single ORF encoded a putative 53.3 kD polypeptide, named Psn6, containing 490 amino acids. [ Conclusion ] RGDV Psn6 exhibited 31.7% amino acid sequence identity to P7 of Rice dwarf virus （RDV）, and 30.0% to P7 of Wound tumor virus （WTV）. This is the first report of the complete sequence of RGDV S6.