热应激预处理对梗阻性黄疸大鼠T细胞亚群的影响

The effect of hyperthermia pretreatment on the subgroups of T lymphocytes in obstructive jaundiced rats

目的:研究热应激预处理对梗阻性黄疸大鼠细胞免疫功能的影响.方法:将30只Wistar大鼠随机均分为3组:假手术组(S组)、胆总管结扎组(B组)、热应激预处理+胆总管结扎组(P组).术后1wk处死大鼠穿刺心脏取血,用流式细胞仪检测各份血液标本的T淋巴细胞(CD4+、CD8+)分类及胆红素含量.结果:胆总管结扎后1wk,大鼠血胆红素明显升高,CD4+减少,CD4+/CD8+下降;热应激预处理组的CD4+、CD4+/CD8+下降不明显,但与胆总管结扎组比较有显著差异(52.60±3.27vs43.52±2.23,2.37vs1.96;P<0.01).鼠T细胞亚响,目前尚关报道.结论:热应激预处理可改善梗阻性黄疸大鼠的细胞免疫功能.

AIM： To study the effect of heat stress pre- treatment on cell-mediated immunity in rats with obstructive jaundice. METHODS： Thirty male Wistar rats were randomly divided into three groups： a sham-operated group （S group）, a bile duct ligation group （B group）, and a heat stress pretreatment ＋ bile duct ligation group （P group）. Each group comprised 10 rats. The rats were sacrificed one week after operation, and the levels of T lymphocytes （CD^4＋ and CD^8＋） and bilirubin in blood samples from the hearts of the rats were measured by flow cytometry. RESULTS： One week after ligation of the common bile duct in rats, the number of CD^4＋ T lymphocytes was significantly decreased compared with that in sham-operated rats, whereas the number of CD8^＋ T lymphocytes was significantly increased. However, the numbers of CD4^＋ and CD8^＋ cells in the P group did not change significantly, which had a statistical difference compared with B group （52.60 ± 3.27 vs 43.52 ± 2,23, 2.37 vs 1.96; P 〈 0,01）. CONCLUSION： Ligation of the common bile duct inhibits cell-mediated immunity in rats. Heat stress pretreatment can improve the cell-mediated immunity in rats with obstructive jaundice.