小熊猫脑源性神经营养因子(BDNF)基因的克隆与序列分析

MOLECULAR CLONING AND SEQUENCING OF THE BRAIN DERIVED NEUROTROPHIC FACTOR(BDNF) GENE FROM LESSER PANDA

参照人的ＢＤＮＦ基因序列设计了一对引物，利用聚合酶链式反应（ＰＣＲ），从小熊猫基因组ＤＮＡ中扩增和克隆到ＢＤＮＦ基因．序列分析表明，小熊猫和人的ＢＤＮＦ基因核苷酸序列同源性为９３％，而与大熊猫ＢＤＮＦ基因的核苷酸序列同源性高达９８％．在推导的多肽序列中，除在前导肽区有两个氨基酸的差异外，小熊猫ＢＤＮＦ的成熟区与人和其他已报道的哺乳动物ＢＤＮＦ成熟区的氨基酸序列完全一致。

The authors employed the polymerase chain reaction (PCR) to amplify the full coding region of the BDNF gene from lesser panda ( Ailurus fulgens) genomic DNA directly with the primers based on the sequence of human BDNF gene.The amplified fragment was cloned and sequenced.Sequence analysis showed that the coding region of BDNF gene from lesser panda was highly homologous with that of human and giant panda counterpart genes (93% and 98% homology,repectively).It was found that there were only four amino acid residues showing difference in the prosequence region by comparing the deduced amino acid sequence of BDNF gene of lesser panda with that of human.However,the amino acid sequence of mature BDNF from lesser panda is identical to that of all the reported mammalian BDNFs.