摘要
目的:探讨丹参对血管紧张素Ⅱ致肾小球硬化的干预作用。方法:采用血管紧张素Ⅱ刺激系膜细胞增殖,同时加入不同剂量的丹参注射液分别对系膜细胞作用24 h。观察系膜细胞纤溶酶原激活剂抑制物-1(plasminogen activator inhibitor-1,PAI-1)mRNA、培养上清液中的PAI-1蛋白和转化生长因子β1(transforming growth factorβ1,TGF-β1)的含量,以及细胞内活性氧(reactive oxygen species,ROS)水平的变化。结果:丹参明显抑制住了由血管紧张素Ⅱ刺激大鼠肾脏系膜细胞而产生的PAI-1 mRNA以及蛋白表达的升高,且其抑制作用的强弱与丹参注射液的剂量密切相关。丹参还明显下调了因血管紧张素Ⅱ刺激后TGF-β1的表达增加以及细胞内ROS水平的升高。结论:丹参下调血管紧张素Ⅱ诱导的系膜细胞PAI-1表达的增加,以及抑制TGF-β1的分泌增加与细胞内ROS水平的增高,从而发挥抗肾小球硬化的作用。
Objective： To investigate the effect of Salvia miltiorrhim on glomeruloscleresis induced by AngⅡ. Method： Rat mesangial cells were exposed to 100 nmol · L^-1 Ang Ⅱ. Meanwhile, we added S. miltiorrhiza injection of different concentrations to Mcs. PAI-1 mRNA and protein, TGF-β1 in serum free MEM medium, and the level of cellular reactive oxygen species （ROS） were measured. Result： S. miltiorrhiza notably attenuated Ang Ⅱ induced expression of PAI-1 in a concentration-dependent manner. Meanwhile, S. militiorrhim suppressed the production of TGF-β1 and cellular ROS in mesangial cells. Conclusion. S. mihiorrhiza can alleviate glomerular sclerosis. The renopretective effects of S. miltiorrhiza may be due to its ability to decrease Ang Ⅱ -induced PAI-1 and TGF-β1 secretion and cellular ROS level.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第22期2400-2404,共5页
China Journal of Chinese Materia Medica
关键词
丹参
纤溶酶原激活剂抑制物
转化生长因子Β1
细胞内活性氧
Salvia miltiorrhiza
plasminogen activator inhibitor-1
transforming growth factorβ1
reactive oxygen species
