摘要
通过人工诱变野油菜黄单胞菌，筛选出1株具良好抗变异性的突变株X．c－18。该突变株能很好地利用蔗糖和玉米淀粉为碳源，蛋白脏和黄豆饼粉为氮源。其最佳黄原胶发酵总糖量与总蛋白质量之比为5∶0．5，最适pH值为7．0，温度为28℃。摇瓶发酵72h，黄原胶产量每升发酵液一般可达30g以上，最高产量达32．6g，对碳源转化率为71．54％。
A genetic stable mutant X. c -18 was induced by UV and nitrous acid treatment from a wild -type Xanthomonas campestris. The mutant could use sucrose and maize starch as the carbon source, peptone and soybean powder as the nitrogen source. The optimum fermentation conditions for xanthan production were: total sugar amount/ total protein amount ratio 5/0. 5. temperature 28℃ and pH 7. 0. over 30 g of xanthan per L fermented broth could be obtained after 72hours shake-flash. The highest xanthan production was 32. 6 g per L broth. The ratio of sucrose utilization was 71. 54%.
出处
《江西科学》
1997年第1期13-20,共8页
Jiangxi Science
