摘要
香菇发酵醪处理液经732阳树脂在pH2．5～3．0时得5’－GMP和5’－CMP混合液，再调至pH4．0～4．5上717阴离子树脂柱，5’－GMP被吸附，用＝3％NaCl溶液解吸，即分离得5’－GMP。醪液经0．2μm胶体磨破壁后分别作了自溶，酶解（5’-磷酸二脂酶）和自溶加酶解三种处理，结果5’－GMP得率分别为94.0％，95．1％和95．0％，未见显著差异。
The mixed solution of 5'-GMP and 5'-CMP could be obtained when Lentinus edodes (Berk)Sing fermented mash is handed by 732 positive Resin at a pH value 2. 5 ~3.0. As pH value was up to 4. 0 ~4. 5 and using 717 negative Resin, 5'-GMP was absorped. Then using 3% NaCl solution as a solvent, the 5'-GMP was separated. Fermented mash was ground by colloid mill to berak the cell wall. After that there were 3 treatments: autolysis, enzyme (phosophodiesterase) digestion and autolysis plus enzyme digestion. The 5'-GMP yield were 94. 0 percent, 95.1 percent and 95. 0 percent respectively, no significamt.
出处
《江西科学》
1997年第1期48-52,共5页
Jiangxi Science
