期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

针对VEGF基因的小分子干扰RNA对子宫内膜癌细胞VEGF基因表达及增殖的影响 被引量：1

原文传递
导出
摘要 RNA干扰（RNA interference，RNAi）是新近发现的一种重要的转录后基因沉默现象，RNAi技术已成为基因功能研究中不可缺少的工具，为治疗病毒感染、肿瘤带来了新的希望。子宫内膜癌是一种严重影响妇女身心健康的恶性肿瘤。血管内皮生长因子（vascular endothelial growth factor，VEGF）是迄今鉴定出来的最重要的血管生成因子，其在恶性肿瘤的发生、发展及预后中具有极其重要的地位，
作者 赵淑萍 马德花 孙芳 姜玉珍 李云芳
机构地区 青岛大学医学院附属医院妇产科
出处 《中华妇产科杂志》 CAS CSCD 北大核心 2007年第11期771-773,共3页 Chinese Journal of Obstetrics and Gynecology
关键词 子宫内膜癌细胞 VEGF基因表达 小分子干扰RNA 血管内皮生长因子 增殖 基因沉默现象 RNAI技术 恶性肿瘤
分类号 R737.33 [医药卫生—肿瘤]
  • 相关文献

参考文献8

  • 1Agami R. RNAi and related mechanisms and their potential use for therapy. Curr Opin Chem Biol, 2002, 6:829-834.
  • 2Hanrahan V, Currie MJ, Gunningham SP, et al. The angiogenic switch for vascular endothelial growth factor (VEGF) -A, VEGF- B, VEGF-C, and VEGF-D in the adenoma-carcinoma sequence during colorectal cancer progression. J Pathol, 2003, 200 : 183- 194.
  • 3Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 ( -Delta Delta C (T)) method. Methods,2001,25:402-408.
  • 4Burger H, Foekens JA, Look MP, et al. RNA expression of breast cancer resistance protein, lung resistance-related protein, muhidrug resistance gene 1 in chemotherapeutic response. ed proteins 1 and 2, and muhidrug breast cancer: correlation with Clin Cancer Res, 2003, 9:827-836.
  • 5李长有,宋今丹,原银栋,范广宇.血管内皮生长因子及其受体在鼠成骨细胞分化中的作用[J].中华实验外科杂志,2004,21(7):852-854. 被引量：13
  • 6Harper SQ, Davidson BL. Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors. Methods Mol Biol, 2005,309:219-235.
  • 7Grzmil M, Thelen P, Hermnerlein B, et al. Bax inhibitor-1 is overexpressed in prostate cancer and its specific down-regulation by RNA interference leads to cell death in human prostate carcinoma cells. Am J Pathol,2003, 163:543-552.
  • 8Filleur S, Courtin A, Ait-Si-Ali S, et al. SiRNA-mediated inhibition of vascular endothelial growth factor severely limits tumor resistance to antiangiogenic thrombospondin-1 and slows tumor vascularization and growth. Cancer Res,2003 ,63 :3919-3922.

二级参考文献8

  • 1Aronow MA,Gerstenfeld LC,Owen TA,et al.Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells[].Journal of Cellular Physiology.1990
  • 2Conn G,Bayne ML,Soderman DD,et al.Amino acid and cDNA sequences of a vascular endothelial cell mitogen that is homologous to platelet-derived growth factor[].Proceedings of the National Academy of Sciences of the United States of America.1990
  • 3Yamane A,Seetharam L,Yamaguchi S,et al.A new communication system between hepatocytes and sinusoidal endothelial cells in liver through vascular endothelial growth factor and Flt tyrosine kinase receptor family (Flt-1 and KDR/Flk-1)[].Oncegene.1994
  • 4Wen Y,Edelman JL,Kang T,et al.Two functional forms of vascular endothelial growth factor receptor-2/Flk-1 mRNA are expressed in normal rat retina[].Journal of Biological Chemistry.1998
  • 5Ohishi K,Ishida H,Nagata T.Thyroid hormone suppresses the differention of osteoprogenitor cells to osteoblasts in cultured rat calvaria cells[].Journal of Cellular Physiology.1994
  • 6Midy V,Plouet J.Vasculotropin/vascular endothelial growth factor induces differentiation in cultured osteoblasts[].Biochemical and Biophysical Research Communications.1994
  • 7Ferrara N.Davis-Smyth T: The biology of vascular endothelial growth factor[].Endocrine Reviews.1997
  • 8Chomczynski P,Sacchi N.Single-step method of RNA isolation by and guanidinium thiocyanate-phenol-chloroform extraction[].Analytical Biochemistry.1987

共引文献12

同被引文献7

引证文献1

中华妇产科杂志
2007年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部