造血干细胞移植预处理诱发口腔黏膜炎模型的建立

Establishment of an animal model of oral mucositis induced by conditioning regimen of haematopoietic stem cell transplantation

目的探讨建立造血干细胞移植预处理诱发口腔黏膜炎大鼠模型的可行性。方法采用马利兰和环磷酰胺预处理方案结合左颊黏膜搔刮建立大鼠口腔黏膜炎模型。设立模型组和对照组,从自然过程、口腔黏膜炎指数、血象、骨髓象、病理检查等指标动态观察口腔黏膜炎的发生、发展过程。结果模型组大鼠于第7天开始出现口腔黏膜炎表现,发生率为80.00%;口腔黏膜炎指数在第11天达到峰值(2.04±1.80),4级以上口腔黏膜炎占39.29%(11/28);第18～21天病损基本恢复,期间大鼠体重持续下降,白细胞计数于第10天下降至最低点,骨髓增生度低下。结论成功建立了造血干细胞移植预处理诱发口腔黏膜炎的大鼠模型。

Objective To establish a rat model of oral mucositis （OM） induced by busulfan and cyclophosphamide （BUCY） conditioning regimen of hematopoietic stem cell transplantation （HSCT）. Methods In the model group, busulfan （6. 0 mg · kg^-1 · d^-1× 4 d） and cyclophosphamide （120 mg · kg^-1 · d^-1× 2 d） were administered by intrastomach perfusion and intraperitoneal injection, respectively. The left cheek mucosas were irritated by superficial scratching on day 6. The oral mucosal score （OMS） was assessed daily. Animals were sacrificed on day 7, 10, 13, 16 and 18. The samples of blood, bone marrow, and the oral mucosa were harvest to evaluate the clinical and histological changes. Results The incidence of oral mucositis in model group was as high as 80. 00% with a survival rate of 73.33%. The initial lesion on the oral mucosa was noted on day 7 with red spot and edema, and then progressive mucositis was characterized by large areas of ulcer formation. The duration of oral mucositis was 8 to 10 days. A continuous weight loss, white blood cell count decrease and bone marrow suppression occurred in the process of oral mucositis. Conclusions An animal model of conditioning regimen-induced oral mucositis was successfully established.