摘要
Background Our previous in vivo study in the rat demonstrates that Shenfu injection, a clinically used extract preparation from Chinese herbs, attenuates neural and cardiac toxicity induced by intravenous infusion of bupivacaine, a local anesthetic. This study was designed to investigate whether bupivacaine could induce a toxic effect in pnmary cultured mouse spinal cord neuron and if so, whether the Shenfu injection had a similar neuroprotective effect in the cell model. Methods The spinal cords from 11 - to 14-day-old fetal mice were minced and incubated. Cytarabine was added into the medium to inhibit the proliferation of non-neuronal cells. The immunocytochemical staining of β-tubulin was used to determine the identity of cultured cells. The cultured neurons were randomly assigned into three sets treated with various doses of bupivacaine, Shenfu and bupivacaine＋Shenfu, for 48 hours respectively. Cell viability in each group was analyzed by methyl thiazoleterazolium （MTT） assay. Results The viability of the cultured neurons treated with bupivacaine at concentrations of 0.01%, 0.02%, 0.04% and 0.08% was decreased in a dose-dependent manner. Although the Shenfu injection at concentrations ranging from 1/50 to 1/12.5 （VN） had no significant influence on the viability of cultured neurons （P〈0.05 vs control）, the injection significantly increased the cellular viability of cultured neurons pretreated with 0.03% bupivacaine （P〈0.05）. Conclusion Although Shenfu injection itself has no effect on spinal neurons, it was able to reduce the bupivacaineinduced neurotoxicity in vitro.
Background Our previous in vivo study in the rat demonstrates that Shenfu injection, a clinically used extract preparation from Chinese herbs, attenuates neural and cardiac toxicity induced by intravenous infusion of bupivacaine, a local anesthetic. This study was designed to investigate whether bupivacaine could induce a toxic effect in pnmary cultured mouse spinal cord neuron and if so, whether the Shenfu injection had a similar neuroprotective effect in the cell model. Methods The spinal cords from 11 - to 14-day-old fetal mice were minced and incubated. Cytarabine was added into the medium to inhibit the proliferation of non-neuronal cells. The immunocytochemical staining of β-tubulin was used to determine the identity of cultured cells. The cultured neurons were randomly assigned into three sets treated with various doses of bupivacaine, Shenfu and bupivacaine＋Shenfu, for 48 hours respectively. Cell viability in each group was analyzed by methyl thiazoleterazolium （MTT） assay. Results The viability of the cultured neurons treated with bupivacaine at concentrations of 0.01%, 0.02%, 0.04% and 0.08% was decreased in a dose-dependent manner. Although the Shenfu injection at concentrations ranging from 1/50 to 1/12.5 （VN） had no significant influence on the viability of cultured neurons （P〈0.05 vs control）, the injection significantly increased the cellular viability of cultured neurons pretreated with 0.03% bupivacaine （P〈0.05）. Conclusion Although Shenfu injection itself has no effect on spinal neurons, it was able to reduce the bupivacaineinduced neurotoxicity in vitro.
基金
This work was supported by a grant from the Ministry of Health of China(No.200312)
