摘要
目的探讨田基黄对人鼻咽癌细胞CNE-2的抑制和凋亡作用,为其临床应用提供实验依据。方法SRB法检测田基黄对CNE-2细胞生长的抑制作用,PI分析细胞周期的变化、Annexin V检测细胞凋亡的发生。结果田基黄抑制CNE-2细胞的生长,引起细胞死亡;田基黄诱导CNE-2细胞发生凋亡和坏死;田基黄作用人鼻咽癌细胞后S期细胞的比例增加,G2/M期细胞的比例减少。结论一定浓度的田基黄可明显抑制人鼻咽癌细胞CNE-2生长,并诱导其凋亡,机制可能与阻滞细胞停留在S期有关。
Objective To explore the effects of Hypericum Japonicum Thunb （HJT） on cell growth inhibition and apoptosis of human nasopharyngeal carcinoma cell line CNE-2 in vitro. Methods Growth inhibition and apoptosis of HJT on CNE-2 cells were measured with SRB assay and flow cytometry respectively. Results The growth of CNE-2 was significantly inhibited by HJT in vitro, and the inhibition was dose-dependent. HJT also induced cell apoptosis and could cause CNE-2 cells to arrest at S phase with the increase of its concentration. Conclusion HJT of definite concentration can evidently inhibit the growth and induce the apoptosis of CNE-2. The mechanism is likely to be associated with the arrest cells at S phase of cell cycle.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2007年第5期337-339,共3页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
江苏省高校自然科学研究指导性计划项目(04KJD320120)
关键词
田基黄
鼻咽肿瘤
细胞凋亡
CNE-2
Hypericum Japonicum Thunb
Nasopharyngeal neoplasms
Apoptosis
CNE-2
