激光照射联合龙葵多糖对荷瘤小鼠肿瘤增殖的影响

Effects of Laser radiation unite Solanum nigtum Polysaccharide onTumor Growth in Cancer Loaded Mice

目的:探讨低能量激光照射联合龙葵多糖对荷肝癌小鼠肿瘤增殖的影响。方法:建立荷肝癌小鼠皮下种植瘤模型,将荷瘤小鼠随机分为5组,连续治疗10d后将小鼠处死。原位杂交法检测瘤组织血管内皮生长因子(VEGF)mRNA表达;免疫组化法检测瘤组织中细胞增殖核抗原Ki67的表达。结果:低能量激光照射联合龙葵多糖可以抑制肿瘤细胞增殖及血管生成,显著下调VEGFmRNA、Ki67的表达。结论:低能量激光照射联合龙葵多糖能抑制肿瘤细胞的增殖及血管生成、抑制荷肝癌小鼠肿瘤的生长。

Objective To study effects of low energy laser radiation united Solanuln nigtum Polysaccarides on tumor growth in liver cancer loaded mice. Method Established liver cancer loaded mouse model and divided randomly mice into five groups, Then calculated the tumor inhibitory rate and spleen index. We detected the expres- sion of VEGFmRNA by in situ hybridization and Ki67 protein by immune histochemistry. Result Low energy laser radiation united Solanum nigtLIlll Polysaccharide could inhibit tumor growth and angiogenesis, the expression of VEGFmRNA, Ki67 protein were decreased. Conclusion Low energy laserradiation united Solanum nigtum Polysaccharide Can obviously inhibit the proliferation of liver cancer cells and angiogenesis, so inhibit the growth of the liver cancer in mice.