胰腺导管腺癌Sp1、PTEN和KLF4的表达及其与微血管密度的关系

Expression of Sp1,PTEN and KLF4 in primary pancreatic invasive ductal adenocarcinoma:Correlation with microvessel density

目的:观察胰腺导管腺癌组织Sp1、PTEN和KLF4的表达,探讨其在肿瘤血管形成中的可能作用。方法:采用免疫组织化学ABC方法,半定量检测30例胰腺导管腺癌组织Sp1、PTEN和KLF4的表达,并选用CD31抗体染色计数肿瘤组织内微血管密度(MVD)。结果:Sp1的阳性表达定位于细胞核;KLF4和PTEN的阳性表达分布于细胞质。20例(67%)有Sp1高表达;20例(67%)有KLF4胞质阳性表达;仅7例(23%)PTEN呈胞质阳性表达。肿瘤细胞的Sp1表达强度与肿瘤组织内MVD值呈正相关(r=0.765,P<0.001),PTEN的阳性表达与MVD值呈负相关(r=-0.465,P<0.05)。Sp1高表达的肿瘤细胞其胞质PTEN和KLF4呈低表达(P<0.001,P<0.05)。结论:Sp1和PTEN与原发性胰腺导管腺癌的肿瘤血管形成密切相关,PTEN和KLF4在胰腺癌中可能起抑癌基因作用。

Objective To explore the expression of Spl, KLF4, and PTEN in pancreatic invasive ductal adenocarcinoma, and clarify their role in tumor angiogenesis of primary pancreatic ductal carcinoma. Methods Surgically resected specimens of 30 primary invasive ductal adenocarcinomas of pancreas were studied by immunohistochemical staining for SP1, KLF4, and PTEN. The MVD was evaluated by CD31 immunostaining. Results Twenty （67%） of the cases showed high SP1 nuclear expression, 20 （67%） of the cases had a high KLF4 cytoplasm reaction, and only 7 （23%） cases were PTEN cytoplasm intensively positive. Positive correlation was found between the SP1 expression and MVD （r=0.765, P〈 0.001）. Negative correlation was detected between the PTEN expression and MVD （r=-0.465, P〈0.05）. KLF4 expression did not show any relationship with MVD. SP1 high expression was associated with a significant lower PTEN and KLF4 expression in tumor tissue （P〈0.001, P〈0.05）. Conclusion Our findings suggest that SP1 and PTEN are involved in the tumor angiogenesis in primary pancreatic ductal adenocarcinomas. PTEN and KLF4 gene might be a tumor suppressor gene for pancreatic carcinomas.