高效液相色谱法同时测定多维营养片中维生素B_6、烟酰胺和泛酸钙

Simultaneous determination of vitamin B_6,nicotinamide calcium and pantothenate by high performance liquid chromatography

目的：建立同时测定维生素补充剂中维生素B6、烟酰胺和泛酸钙含量的液相色谱法。方法：样品在提取液中经超声提取和水浴加热溶解后,以0.05 mol/L磷酸二氢钾溶液（pH=3.00±0.05）-甲醇-乙腈（90：6：4）为流动相,经In-ertsil PH-3苯基柱分离,在波长210 nm处检测,外标法定量。结果：维生素B6、泛酸钙和烟酰胺分别在0.0024～0.060 mg/ml（r=0.9999）、0.0080～0.20 mg/ml（r=0.9999）、0.017～0.42 mg/ml（r=0.9999）浓度范围内呈良好的线性关系,维生素B6、泛酸钙和烟酰胺的平均回收率分别为97.2%、99.9%、101.2%。结论：该方法简便、准确、重现性好,适用维生素类保健食品的日常质量控制。

Objective：To develop a method for simultaneous determination of vitamin B6,calcium pantothenate and nicotinamide in multivitamin supplements by high performance liquid chromatography and establish a simple, accurate and reliable method for determination of the assay of multivitamin supplements. Methods： The samples were dissdlved in extracted solution and then prepared by ultrasonic extraction and water heating in the first step. And the chromatographic conditions were as follow：Inertsil PH - 3 column （250 × 4. 6 mm, 5 μm） was the analytic column ; the mobile phase were 0.05 mol/L potassium dihydrogen phosphate （ pH = 3.00 ±0.05 ） methane - acetonitrile （ 90 ： 6 ： 4 ） ; UV detection was performed at 210 rim. Results ： The standard curves for vitamin B6, calcium pantothenate and nicotinamide were linear in the range of 0. 0024 -0. 060 mg/ml （r = 0. 9999, n = 6） ,0. 0080 - 0. 20 mg/ml （ r = 0. 9999, n = 6） ,0. 017 - 0. 42 mg/ml （ r = 0. 9999, n ： 6）, respectively. The average recoveries of vitamin B6 ,calcium pantothenate and nicotinamide at different concentration were 97. 2%, 99. 9% and 101.2%, respectively. Conclusion ：The method is convenient, accurate and precise. It can be used to control the quality of multivitamin health foods.