二极管阵列检测器和荧光检测器联合测定饮用水中多环芳烃

Determination of polynuclear aromatic hydrocarbons in drinking water by high performance liquid chromatography/series of photo-diode array detector and fluorescence detector

目的：探讨二极管阵列检测器（DAD）和荧光检测器（FLD）联用,高效液相色谱法测定饮用水中多环芳烃的应用。方法：进行二极管阵列检测器（DAD）和荧光检测器（FLD）联用分离条件和样品处理试验,得出最佳色谱条件。结果：选定条件下,6种多环芳烃在测定范围内线性关系良好,相关系数0.9935～0.9999;荧蒽、苯并（b）荧蒽、苯并（k）荧蒽、苯并（a）芘、苯并（ghi）芘、茚（1,2,3-CD）芘方法检出限分别为1.40,1.73,1.01,2.46,1.83,8.28（ng/L）;回收率70%～110%。结论：DAD和FLD联用具有较强定性能力,重现性好,灵敏度高,成功应用于饮用水中多环芳烃监测。

Objective ：To explore the application of detecting polynuclear aromatic hydrocarbons （PAHs） in drinking water by high performance liquid chromatography（ HPLC）/series of photo - diode array detector（DAD） and fluorescence detector（FLD）. Methods：Extracting water samples and detecting the extraction by HPLC/series of DAD and FLD. Result： Under the detecting condition, the linearities of six kinds of polynuclear aromatic hydrocarbons were good in the range of detection. And the correlations were at 0. 9935 ～0. 9999, the recovery rates were 70. 0% ～ 110. 0%, the limits of detection were：fluorescence 1.40 mg/L, benzo （b） fluorescence 1.73 ng/L, benzo （k） fluorescence 1. O1 ,benzo （a） pyrene 2.46 ng/L,benzo （ghi） perylene 1.83 ng/ L,indeno （ 1,2,3 - cd） pyrene 8.28 ng/L Conclusion： By using series of DAD and FLD, it has got a good qualitation, repeatability and sensitivity of determination. The method has been applied to monitering polynuclear aromatic hydrocarbons in drinking water successfully.