石墨炉原子吸收法直接测定血清中硒的方法研究

Determination of selenium in serum by electrothermal atomic absorption spectrometry

目的：建立测定血清中硒的新方法。方法：应用Triton X-100稀释样品,横向塞曼石墨炉原子吸收光谱法直接测定样品中痕量硒。结果：选定了最佳测量条件,该方法的灵敏度、精密度均能满足检测工作的需要。本法对硒的最低检出量为14.7 pg,回收率在90%～102%之间。结论：本法简便、快速、准确,灵敏度和精密度高,适用于血清中硒的测定。

Objective ：To establish a new method for determination of selenium in serum. Methods ：The samples were diluted in a medium containing Triton X - 100 before being introduced directly into the furnace and then detected by Zeeman GFAAS. Results：Under the best condition, the sensitivity and precision were suitable for the determination while the rate of recovery was controlled between 90% to 100%. The lowest detection limit of serum was 14. 7 pg. Condusion：This method is simple, quick, accurate and sensitive. It is a very good method for measuring selenium in serum.