摘要
目的:建立气相色谱法测定尿中盐酸氯丙嗪分析方法。方法:用乙酸乙酯为萃取剂,通过液-液萃取尿液中微量盐酸氯丙嗪,在DB-1701石英弹性毛细管柱上分离,氮磷检测器检测。实现中毒病人尿液中盐酸氯丙嗪的快速检测。结果:乙酸乙酯萃取效果好,方法最小检出浓度0.05μg/ml、相关系数0.9993、变异系数3.05%~4.15%、样品加标回收率98.1%~101%。结论:方法准确性好、灵敏度高、简便、快速,适合盐酸氯丙嗪中毒事件的快速检测。
Objective:To establish a analytic method for detection of chlorpromazine hydrochloride in urine by gas chromatograph spectrometry. Methods:The liquid samples were extracted from urine by ethyl acetate. The extractive solution was analyzed by using GC - NPD and DB1701 column. Results:Expression effects were shown in the liquid - liquid extraction. The limit of detection for chlorpremazine hydrechloride was 0. 05μg/ml,with the relative Coefficients of 0. 9993. The variability coefficient ranged from 3.05% ~ 4. 15%. The recoveries with standard addition ranged from 93.6% ~ 96.4%. Conclusion: The methods is accurat, highly sensitive, convenient and fast. It is suitable for quick dectection of chlorpremazine hydrechloride poisoning.
出处
《中国卫生检验杂志》
CAS
2007年第11期2003-2004,共2页
Chinese Journal of Health Laboratory Technology