合肥市屠宰生猪肉样沙门菌的PCR快速检测

Rapid detection of Salmonella from pork samples in Hefei by PCR

目的:了解合肥市屠宰生猪肉样中沙门菌的污染状况。方法:应用聚合酶链式反应(polym erase chain reaction,PCR)对200份生猪肉样进行沙门菌的快速检测,并与常规法检测结果以及国内其他11个城市生猪肉样沙门菌的检出率作比较分析。结果:200份生猪肉样中沙门菌检出率为20%(40/200),污染率处于较高水平,而常规法的检出率为13%(26/200)。结论:合肥市生猪肉样中沙门菌的污染状况较为严重,存在发生食源性沙门菌病的潜在危险,PCR技术对沙门菌检测时间仅需2 d,与常规法相比,快速、简便、准确的优势特点使其成为调查食源性沙门菌的有用工具。

Objective：To understand the contamination of Salmonella in pork in Hefei. Methods：A total of 200 pork samples were detected rapidly by polymerase chain reaction （PCR）. The detective rate was also evaluated as compared with the detective rate by national standards and the detective rate from eleven cities. Results ：The forty SalmoneUa strains were isolated in 200 pork samples and the positive rate was 20% , but the positive rate was 13% based on criteria of national standards. The contamination of Salmonella in pork in Hefei was at a high level. Conclusion ：The contamination of Salmonella in pork in Hefei is serious. There is possibility and potential danger of Salmonellosis. The detection time of Salmonella by PCR is two days. As compared with national standards, PCR is an useful method of investigating food - borne Salmonella because of its rapid, convenient and accurate characteristics.