分娩发动前后外周血和胎盘中人类白细胞抗原G1的变化及其意义

Change of HLA-G1 levels in placenta and peripheral plasma before and after the onset of normal

目的研究足月妊娠分娩发动前后外周血和胎盘局部人类白细胞抗原G1(HLA-G1)变化并探讨其在分娩发动中的作用。方法应用ELLISA方法检测孕晚期未临产组(剖宫产组)和临产组外周血中人类白细胞抗原G1(sHLA-G1)浓度,并通过RT-PCR法比较两组胎盘组织中HLA-G1mRNA水平。结果与未临产组相比,临产组血浆中sHLA-G1表达强度明显下降,差异有统计学意义(P<0.01),临产组胎盘局部中HLA-G1 mRNA数量也明显低于孕晚期未临产组(P<0.01)。结论分娩发动时胎盘合成和分泌HLA-G1减少导致局部和外周血中sHLA-G1下降,可能激活多种免疫细胞并调节免疫细胞分泌细胞因子,导致循环及胎盘局部各种炎性细胞因子释放增加,使母胎界面细胞因子分泌增加,从而参加了分娩。

Objective To investigate the change of HLA-G1 levels in placenta and peripheral plasma before and after the onset of normal labor in order to understand the role of HLA-G1 in initiating the normal labor. Methods ELLISA was used to detect the sHLA-G1 in peripheral plasma of the women with late pregnancy before （surgical labor） or after （natural labor） the onset of labor. Semi quantitative reverse transcription polymerase chain reaction （RT-PCR） was used to measurethe mRNA level of HLA-G1 in placenta. Results Compared with the levels of HLA-G1 mRNA and sHLA-G1 in the surgical labor group, the levels of HLA-G1 mRNA and sHLA-G1 decreased in the natural labor group （ P 〈 0.01 ）. Conclusion When the labor initiates, the placenta decreases the secretion of HLA-G1, which activates multiple immune cells to secrete cytokines that stimulate the release of various inflammatory mediators taking part in the normal labor.