麦冬药材及其提取物中甲基麦冬黄烷酮A和B的HPLC法测定

Determination of methylophiopogonanones A and B in Radix Ophiopogonis and its extracts by HPLC

目的测定麦冬药材及其提取物中甲基麦冬黄烷酮A(MOA)和甲基麦冬黄烷酮B(MOB)的量,为麦冬药材及其提取物的质量控制提供科学依据。方法采用HPLC-UV法测定麦冬药材和提取物中MOA和MOB的量。色谱条件:Kromasil C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水(55∶45);体积流量:1mL/min;检测波长:298nm;柱温:30℃。结果川麦冬和浙麦冬药材含MOA的量分别为0.0040%～0.0096%、0.0067%～0.0134%,MOB的量分别为0.0021%～0.0062%、0.0159%～0.0282%;川麦冬和浙麦冬提取物中MOA的量分别为0.0075%～0.0088%、0.0113%～0.0126%,MOB的量分别为0.0038%～0.0051%、0.0207%～0.0238%。结论浙麦冬药材和浙麦冬提取物中MOA和MOB的量均分别高于川麦冬药材和川麦冬提取物;该方法可为麦冬药材及其提取物的质量控制提供参考。

Objective To determine the contents of methylophiopogonanone A （MOA） and methylophiopogonanone B （MOB） in Radix Ophiopogonis and its extracts. Methods An HPLC-UV method was used for determining the contents of MOA and MOB in all samples. Analytical column was Kromasil C18 （250 mm×4.6mm,5μm）. Mobile phase was acetonitrle-water （55 ： 45） and detection wavelength was 298 nm. The flow rate of mobile phase was 1 mL/min, and temperature was 30 ℃. Results The contents of MOA in Radix Ophiopogonis cropped in Sichuan and Zhejiang Provinces were 0. 004 0%- 0. 009 6%, 0. 006 7%-0. 013 45, and the contents of MOB were 0. 002 1%-0. 006 2%, 0. 015 9%-0. 028 2%, respectively. The contents of MOA in the extract of Radix Ophiopogonis cropped in Sichuan and Zhejiang Provinces were 0. 007 5%-0. 008 8%, 0. 011 2%-0. 012 6%, and the contents of MOB were 0. 003 8%_ 0. 005 1%, 0. 020 7%-0. 023 8%, respectively. Conclusion The contents of MOA and MOB in Radix Ophiopogonis cropped in Zhejiang Province and its extracts are more than those in Sichuan Province and its extrouts. The method can be used for the purpose of the quality control of Radix Ophiopogonis and its extracts.