不同卵密度与培养条件对夜蛾斯氏线虫侵染期幼虫形成的影响

Effect of Egg Densities and Cultural Conditions on the Formation of Steinernema feltiae Infective Juveniles

利用24孔培养板研究不同营养水平、pH值、温度和卵密度对夜蛾斯氏线虫侵染期幼虫形成的影响。结果表明：在Ringer溶液中,无论卵密度高低,卵或初孵幼虫均很快死亡;在10%基本培养液营养水平条件下线虫的发育停滞在二龄阶段;20%-100%的基本培养液营养水平下,侵染期幼虫的形成比率同营养水平呈负相关,与线虫卵密度呈正相关;适宜线虫向发育期三龄幼虫发育的pH值为7-8,温度为20-25℃,高于或低于此pH值或温度范围均可促使侵染期幼虫的形成;夜蛾斯氏线虫在pH值小于5或温度高于30℃条件下会很快死亡。

The effects of nutrition, pH, temperature and egg densities on the infective juvenile formation of Steinemema feltiae were evaluated in 24-well culture plates. In the Ringer solution, all the eggs or juveniles died; at 10% strength of nematode culture medium, nematodes stayed in the second stage after hatching from the eggs. At above 20% strength of nematode culture medium, the formation of infective juveniles were negatively related to nutrition strength and positively related to egg densities. The optimum conditions for third stage nematode development were pH 7 - 8 and 20 - 25 ℃, higher percentage of infective juveniles would form out of those ranges. When pH was less than 5 or temperature was higher than 30 ℃, the nematodes would die.