摘要
线粒体DNA指纹是指线粒体DNA控制区内的高变异区碱基突变导致的多态、重复序列数目的变异导致的异质型多态和分子长度多态。试验根据牛线粒体DNA非编码区D-loop环全长序列和编码区细胞色素B(Cyt B)基因部分序列,研究在日本黑牛和延边黄牛、鲁西黄牛群体中碱基由于发生倒换、颠换、插入、缺失形成的DNA多态性。结合生物信息学方法分析,发现在15头日本黑牛群体中489号个体D-loop全序列存在44处特异性位点,日本黑牛489号和120号Cyt B部分序列存在4处特异性位点。构建分子进化树结果表明:日本黑牛和延边黄牛存在较近的亲缘关系;日本黑牛489号、120号个体与鲁西黄牛中D-loop单倍型H18、H19、H21有较近的亲缘关系,120号Cyt B的单倍型又与鲁西黄牛共享1个单倍型Hap4,120号与鲁西黄牛存在更近的亲缘关系。
Mitochondrial DNA fingerprinting focus on polymorphisms contributed to nucleotide variauce and number variation that altered in mitochondrial DNA hypervariable region. Two specific haplnids were found in 15 Japanese Black Boville. The homology analysis showed that their blood relationship is closer with Luxi Bovine, cluster analysis and eladogram showed Hap4 was clustered with Hap6. Japanese Bovine 489 and 120 are closer in maternal line blood relatinnship with Luxi Bovine D -loop haplotypes H18, H19, H21 ,and 120 is more closer in bloodrelatlonship with l,uxi Bovine
出处
《黑龙江畜牧兽医》
CAS
北大核心
2007年第11期15-17,共3页
Heilongjiang Animal Science And veterinary Medicine
