摘要
免疫细胞化学和电镜观察人乳头瘤病毒（ＨＰＶ）形态发生结果表明：该病毒抗原见之于棘层、颗粒层和角质层细胞核内；毒粒则仅见之于颗粒层及角质层。从而提示了棘层以上的细胞层次为ＨＰＶ复制、装配和发育成熟的主要场所。本研究还观察到了ＨＰＶ装配的过渡阶段，宿主细胞的“溶解”及含有ＨＰＶ颗粒的细胞不一定都出现空泡等现象，从而提示了该病毒可因细胞溶解而释放，并造成上皮内扩散和再感染；空泡细胞不能作为诊断该病毒感染的可靠依据。
The pointed warts of female genital were studied with the techniques of immunocytochemisty and electron microscope respectively for the morphogenesis of human papillomavirus (HPV). The results of immunocytochemistry study showed that HPV capsid antigen was present at nuclei of spinous, granular and horny cells in the epithelium of warts. However, the virus particles were only found in the granular and horny cells under electron microscope. These findings suggest that there is a close association between HPV morphogensis and differentiation of keratinized epithelium. In addition, most of the cells with a HPV antigen positive mucleus had no hollow in their cytoplasm, while only a few of those cells called “koilocyte” did.
