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实时定量PCR技术在瘤胃微生物定量分析中的研究应用 被引量：2

Application of Real-Time PCR Approach for Quantification and Analysis of Ruminal Bacteria
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摘要 随着对瘤胃微生物研究的逐步深入,一些以分子生物学技术为基础的定量分析方法对瘤胃微生物的研究方兴未艾。本文简要介绍了实时定量PCR技术的原理、操作程序以及该技术在反刍动物瘤胃微生物定量分析中的应用现状、取得的成果和前景展望。 With the studying of ruminal bacteria becoming intensive gradually, some methods of quantitative analysis based on molecular biology technology are in the ascendant for studying rumen microbe. This paper illustrated the theory, operation process of real-time PCR and its actuality of application, achievement and prospect in future.
作者 刘大程 张迪 卢德勋 高民 孙海洲
机构地区 内蒙古农业大学动物科学与医学学院 内蒙古畜牧科学院
出处 《中国畜牧杂志》 CAS 北大核心 2007年第21期51-53,共3页 Chinese Journal of Animal Science
基金 国家自然科学基金(30460095 30560105)
关键词 REAL-TIME PCR 瘤胃微生物 综述 定量 real-time PCR ruminal bacteria a review quantification
分类号 S816.3 [农业科学—饲料科学]
  • 相关文献

参考文献10

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二级参考文献15

  • 1Russell J B,Rychlik J L.Factors that alter Rumen microbial ecology.Science,2001,292:1119-1122.
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共引文献19

同被引文献48

引证文献2

二级引证文献4

中国畜牧杂志
2007年 第21期

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