肝癌组织中N-ras基因突变和p53蛋白表达

Analysis of N ras gene mutation and p53 expression in human hepatocellular carcinoma

目的：研究Ｎ－ｒａｓ和ｐ５３基因与肝癌发生、发展的关系。方法：应用多聚酶链反应－单链构象多态性分析法（ＰＣＲ－ＳＳＣＰ）和免疫组化法，检测２９例肝癌中的Ｎ－ｒａｓ基因突变和ｐ５３蛋白的表达。结果：１３例肝癌ｐ５３蛋白阳性（４４．８％），表明广西地区肝癌中较普遍存在ｐ５３基因的突变。肝癌及癌旁组织中Ｎ－ｒａｓ基因在第２～３７密码子间的突变率分别为７９．３１％和８０．７７％，２２例有２个以上突变位点（７５．８６％）。１２例（４１．３７％）肝癌可同时检出Ｎ－ｒａｓ基因突变和ｐ５３蛋白阳性。在３８％的肝癌中虽有Ｎ－ｒａｓ基因突变，却呈ｐ５３蛋白阴性。结论：以上两种基因可能协同参与肝癌的发生和发展。同时可能还有其他基因或因子参与。

Purpose To study the relationship between N ras gene mutation and p53 gene expression and the carcinogenesis and the development of human hepatocellular carcinomas. Methods The mutations of N ras gene and the p53 gene expression were analyzed in 29 cases of hepatocellular carcinomas(HCCs) by polymerase chain reaction single strand conformation polymorphism(PCR SSCP) and immunohistochemistry. Results Thirteen cases of HCCs were p53 positive(44 8%), which showed a rather high percentage of p53 gene mutation in Guangxi. The aberrations at N ras codon 2￣37 were found in 79 31% of HCCs and 80 77% of adjacent non tumour liver tissues. More than 2 point mutations of N ras gene were observed in 22 cases (75 86%). Twelve cases(41 37%) of HCCs showed both N ras gene mutation and p53 gene expression. Conclusions The results suggest that N ras gene and p53 gene may involve in the carcinogenesis and the development of HCC. It is notable that 38% of HCCs with N ras gene mutation did not express p53 protein, which indicates that other genes or factors may play roles in the carcinogenesis and the development of HCC.