摘要
应用酶动力学抑制实验，观察了７种药物对牛睾丸纯化的醛糖还原酶的抑制作用，结果发现ｓｏｒｂｉｎｉｌ，ｔｏｌｒｅｓｔａｔ，水飞蓟宾，黄连素酶抑制作用较强，其抑制常数分别为：１０８，０．１２μｍｏｌ／Ｌ；３５，２７μｇ／ｍｌ。另外我们认为，利用本实验建立的方法可以进行醛糖还原酶抑制剂的初步筛选。
The inhibitory effects of seven agents on aldose reductase were studied by enzymatic kinetics using the purified enzyme from bovine testis. sorbinil, tolrestat, silybin and berberini were found to exhibit a marked enzyme inhibitory effect with Ki values of 1.08μmol/L,0.12μmol/L,3.5μg/ml,and 2.7μg/ml respectively.The established assay system described in this paper may be useful in screening aldose reductase inhibitors.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1997年第6期372-373,共2页
China Journal of Chinese Materia Medica
关键词
糖尿病
醛糖还原酶
抑制剂
diabetes
aldose reductase
aldose reductase inhibitors
