摘要
AFB1和HBV是致肝癌的两个主要因素。研究表明AFB1与HBV有协同致肝癌作用,但其机理不明。方法利用HBV转基因小鼠模型,分析小鼠肝脏组织中的细胞色素P450酶的含量和活性,GSH和GST的含量和活性,并测定HBV转基因小鼠暴露于AFB1后血清AFB1┐白蛋白加成物的含量。结果加成物的含量在HBV转基因小鼠都较对照小鼠高,细胞色素P450酶含量明显降低而活性有所上升,GSH含量和GST酶活性都明显下降。结论HBV转基因小鼠的解毒代谢能力下降。HBV影响了小鼠的解毒代谢,使小鼠对AFB1敏感。
Aflatoxin B1 (AFB1)is activated to form AFB1 8,9 epoxide via cytochrome P450s.The epoxide can then be rendered innocuous via phase 2 enzymes(glutathione S transferases GSTs). The mechanistic role of human hepatitis B viurs(HBV) in liver tumorigenesis is unresolved.Methods:To determine whether HBV transgenic mice are more susceptible to the effects of AFB 1,livers from transgenic and control non transgenic mice were analyzed for the enzyme systems involved in metabolism. Results: The levels of liver cytochrome P450s and GSH and the activity of GSTs of the transgenic mice were significantly lower than nontransgenic mice( P <0 01).The activities of cytochrome P450 enzymes in the livers of transgenic mice were higher than those of non transgenic mice.After exposure to AFB1, the content of AFB1 albumin adducts in the sera of transgenic mice were higher than those of non transgenic mice at multiple timepoints over 24 hours. Conclusions:These data suggest that HBV decreases detoxification of AFB1 in the transgenic mice and are consistent with the synergy observed between HBV expression and hepatocarcinogenesis in animal models and epidemiological studies.
出处
《肿瘤》
CAS
CSCD
北大核心
1997年第4期187-189,共3页
Tumor
基金
国家自然科学基金癌基因和相关基因国家重点实验室基金资助国家九.五攻关基金
关键词
代谢解毒
黄曲霉毒素
HBV
肝肿瘤
Metabolic detoxication Aflatoxin B1 Hepatitis B virus,Transgenic mice