细菌激活系统血液免疫反应仿真自然体系实验研究

Experimental study on systemic hematogenic immunoreactions activated by bacteria in simulation of natural system

目的：用现代系统免疫学仿真自然体系探讨血液细菌感染的系统血液免疫反应状况。方法：将酵母菌及灭活卡介苗（5×10^8／mL）0．2mL分别激活全血0．2mL或WBC 0．2mL和血浆（或0．9％氯化钠溶液）0．3mL，37℃水浴1h，用流式细胞仪法（FCM）、ELISA等方法观察IL-8含量，补体C3、C4和趋化因子受体Fy6的变化。结果：在血浆存在的条件下，细菌可激活RBC调控和促进WBC产生IL-8，使自然实验组IL-8激活指数（37．04±34．84）明显高于WBC激活分离实验组IL-8激活指数（1．09±0．77），而且前者补体叫增值（0．0100±0．0100）也明显高于后者（-0．0027±0．0080），差异有统计学意义（P〈0．05）。结论：酵母菌及灭活卡介苗在血液中先被补体系统识别、激活，绝大部分附有C3b的细菌被红细胞黏附；提呈给WBC后，町引发血浆免疫反应、RBC免疫反应、吞噬细胞免疫反应和淋巴细胞免疫反应。

Objective To study systemic hematogenic immunoreactions induced by bacterial infections using simulation of natural system. Methods Whole blood 0.2 mL or white blood cells 0.2 mL and plasma（or normal saline） 0. 3 mL were stimulated by 0. 2 mL of yeast and inactivated Bacillus Calmette-Guerin（BCG, 5 X 108/mL）, respectively, which were incubated at 37 ℃ for 1 h. Interleukin（IL） -8, C3, C4 and chemokine receptor Fy6 were detected by flow cytometry （FCM） and enzyme-linked immunosorbentassay（ELISA）. Results Bacteria could activate red blood cell to modulate IL-8 release from white blood cells in plasma. In nature experimental group, activation rate （37.04 ± 34.84） of IL-8 was significantly higher than that （1.09 ± 0.77） in isolation experimental group. In nature experimental group, value increment （0.01± 0. 01） of complement C4 was significantly higher than that （ 0. 002 7 ±0. 008） of isolation experimental group（P 〈 0.05）. Conclusions Yeast and inactivated BCG are recognized by complements in the blood. Most bacteria adhered with C3b are attached on red blood cells, and then presented to white blood cells, which induces plasma immunoreactions, red blood cell immunoreactions, phagocyte immunoreactions and lymphocyte immunoreactions.