竹节参总皂苷对大鼠局灶性脑缺血损伤及抗氧化酶活性的影响

Effects of Total Rhizoma Panacis Japonica Saponins on Injury and Antioxidase Activities in Rats Brain with Focal Cerebral Ischemia

目的研究竹节参总皂苷(total rhizoma panacis japonica saponins,tRPJS)对大鼠局灶性脑缺血损伤的保护作用及机制。方法插线法阻塞大鼠右侧大脑中动脉24h后,观察tRPJS对模型大鼠病理形态学及缺血脑组织谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及丙二醛(MDA)含量的影响。结果竹节参总皂苷(200,100,50mg·kg-1)组大鼠脑组织的病理改变较模型组有不同程度的减轻,缺血侧脑组织抗氧化酶GSH-PX、SOD、CAT活性显著升高,MDA的含量明显降低。结论竹节参总皂苷抗脑缺血损伤作用机制可能与其改善机体自由基代谢有关。

Objective To investigate the protective effects of total rhizoma panacis japonica saponins （tRPJS）on rat brains after focal cerebral ischemia injury, and to explore its protective mechanisms of tRPJS. Methods Focal cerebral ischemia in rats was produced by 24 h occlusion the right part of the middle cerebral artery（MCAO）. The activities of glutathione peroxidase （GSH - PX）, superoxide dismutase （SOD）, catalase （CAT） and malondialdehyde （MDA） in ischemic tissue of brain as well as the brain pathological changes were analyzed. Results The activities of GSH - PX, SOD and CAT were lowered and the content of MDA was elevated in the model rats. Treatment with tRPJS 200, 100, 50 mg·kg^-1 could significantly improve the activities of all the enzymes mentioned above and reduce the content of MDA in ischemic tissue of brain. Conclusion The tRPJS has protective effect against focal cerebral ischemic injury and this may be related to the increase of activities the GSH - PX, SOD and CAT and to the reduction of the lipid peroxidation.