泽泻饮片的HPLC-ELSD指纹图谱研究

Development of Chromatographic Fingerprint of Rhizoma Alismatis Orientalitis by HPLC-ELSD

目的建立泽泻饮片甲醇提取液的高效液相指纹图谱(HPLC-FP),为其鉴别提供依据。方法在Dia-monsil TM C18柱(250mm×4.6mm,5μm)上以甲醇-水为流动相进行梯度洗脱,流速:0.5mL·min-1;记录时间70min;蒸发光散射检测器(ELSD)检测;检测条件:漂移管温度82.5℃,雾化气体流速1.9L·min-1;实验数据用聚类分析和相似度软件处理。结果得到15个共有峰,聚类分析和相似度分析结果显示16批泽泻饮片的指纹图谱存在差异。结论结果重现性好,为控制泽泻饮片的质量提供了可靠的分析方法。

Objective A fingerprint of Rhizoma Alismatis Orientalitis＇decoction pieces was established by HPLC- ELSD. Methods The gradient elution mode was applied in chromatographic separation by mobile phase of methanol and water at flow rate of 0.5 mL·min^-1 in a Diamonsil TM Cls chromatographic column （250 mm × 4.6 mm, 5 μm）, and recording time was 70 minute. The parameters of evaporative light scattering detector were set as follows： the temperature of the drift tube was 82.5 ℃ and gas flow rate was 1.9 L · min^-1. The data was analyzed with cluster analysis and similarity calculation software. Results The fingerprint showing 15 common peaks was obtained. The results of cluster analysis and similarity calculation showed there existed differences in fingerprints of 16 batchers of medicinal slices of Rhizoma Alismatis Orientalitis. Conclusion The method is effective and reproducible for the quality control of medicinal slices of Rhizoma Alismatis Orientalitis.