马钱子总生物碱的含量测定

Quantitative Determination of Total Alkaloids from Seeds of Strychnos nux-vomica L.

目的建立马钱子总生物碱的含量测定方法并用于提取工艺的评价。方法采用酸性染料比色法测定总生物碱含量:以马钱子碱和士的宁为对照品,溴麝香草酚蓝为酸性染料,在pH7.2缓冲溶液条件下,用氯仿萃取。结果马钱子碱在0.0412～0.206mg/mL范围内,士的宁在0.0428～0.214mg/mL范围内浓度与吸光度值呈良好的线性关系。马钱子碱的平均回收率为(101.8±4.6)%(n=5);士的宁的平均回收率为(103.3±2.7)%(n=5)。结论所建立的总生物碱测定方法简便、准确、稳定,可以满足马钱子总生物碱提取工艺考察的需要。

Objective To establish the method for the determination of the content of total alkaloids extracted from seeds of Strychnos nux - vornica L. Methods The content of total alkaloids in the samples was determined by the acid dye colorimetry, which was performed with brucine and strychnine as the reference substances, dibromothymolsuffonphthalein as the acidic dye under the condition of a buffer solution （pH = 7. 2）, and chloroform was used as the extractant. Results Both the concentration of brucine being 0. 041 2 - 0. 206 mg/mL and that of strychnine being 0. 042 8 - 0. 214 mg/mL had a good linear relationship with the absorbance. The average recoveries for brucine and strychnine were （101.8 ± 4.6） % and （103.3 ± 2.7） % , respectively. Conclusion The established method is simple, accurate and stable, and can be used for the determination of the content of the total alkaloids extracted from the seeds of Strychnos nux - vornica L.