摘要
目的建立木豆叶中牡荆苷的含量测定方法。方法色谱柱为Hypersiol ODS2 C18柱(250mm×4.6mm,5μm);流动相:0～20min,甲醇∶1%醋酸(25∶75),20min后,甲醇∶1%醋酸(30∶70);流速:1.0mL/min;检测波长:339nm。结果牡荆苷在0.1044～0.522μg范围内线性关系良好,r=0.9994,平均回收率为100.11%,RSD为0.63%。结论该法简便,重复性、稳定性良好,可作为木豆叶中牡荆苷含量测定方法。
Objective To develop a quantitative method to determine vitexin in Cajanus cajan （L.） Millsp. Methods The chromatographic conditions were as follows： column C is （250 mm × 4.6 mm, 5 μm） , mobile phase being MeOH ： 1% HAC （25 ： 75） for 20 mins and then being MeOH ： 1% HAC （30 ： 70） after 20 mins, flow rate at 1.0 mL/min, and wavelength at 339 nm. Results The good linearity of this method was in the range of 0. 104 4 - 0. 522 μg （ r = 0. 999 4）, and the average recovery of vitexin was 100. 11% （ RSD = 0.63 % ）. Conclusion The method is simple and sensitive with good stability, it can be suitable for the quality control of vitexin in Cajanus cajan （L.） Millsp..
出处
《中药新药与临床药理》
CAS
CSCD
2007年第6期474-476,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
木豆叶
牡荆苷
HPLC
含量测定
Cajanus cajan （L.） Millsp.
Vitexin
HPLC
Content determination
