3种十字花科植物花期若干生化指标的比较

Comparative Study on Some Biochemical Index of Three Cruciferae Plants

测定了十字花科植物荠菜、独行菜、播娘蒿不同器官的过氧化物酶(POD)、α-淀粉酶和苯丙氨酸解氨酶(PAL)的活性以及可溶性蛋白和过氧化氢(H2O2)的含量,同时采用聚丙烯酰胺凝胶电泳方法,对过氧化物酶及α-淀粉酶同工酶进行了分析。结果表明:3种植物不同器官的POD、α-淀粉酶、PAL活性和可溶性蛋白、H2O2含量存在差异,POD和α-淀粉酶同工酶谱条带在不同器官之间也存在着差异,有可能POD和PAL的活性与植物的药用成分之间存在一定的联系。

The activities of peroxidase （POD）, α-amylase and phenylalanine ammonia-lyase （PAL） as well as the contents of soluble protein and hydrogen peroxide （H2 O2 ） in different or- gans of the three plants[Capsella bursa-pastoris （L.） Medic,Lepidiurn apetalurn Willd, and De- scurainia sophia （L.） Webb] were investigated. Simultaneously,the patterns of peroxidase and α - amylase isozyme were analyzed by PAGE. The result indicated that there were some differences in the activity of POD,α-amylase,PAL and the content of H2O2 in different organs;and the patterns of POD and α - amylase isozyme were different too. The activities of POD and PAL might be related to the medicinal component of plants.