摘要
应用免疫组化LSAB法对52例胃癌患者检测耐药相关蛋白（MRP）在胃癌组织中的表达。结果发现52例胃癌中20例阳性，阳性表达率为38．5％。MRP在肿瘤细胞膜上和胞浆内均有表达，且以胞浆内（粗颗粒状）更明显。晚期胃癌（Ⅲ、Ⅳ期）阳性率为60％，显著高于Ⅰ、Ⅱ期早期胃癌的18．5％表达率（P＜0．01）。MRP阳性者平均生存期（20．9±20．7月）和5年生存率（10％）显著低于阴性者（分别为48．5±22．7月与65．6％，均P＜0．01）。提示，MRP在胃癌中的表达与预后密切相关，其可能是内源性耐药的重要机制之一。
LSAB immunocytochemical staining using MRP-specific Mab QCRL- 1 in formalin - fixed, paraffin- embedded carcinoma tissue was performed to determin whether immunostaining correlated with tumor types, grade, depth of invasion, lymph node metastasis and clinical prognosisIn 50 cases 20 positive staining were found which was detected in the cell cytoplasma as well as on plasma membrane. The results showed that the expression of MRP was higher in patients with advanced disease as compared with those having early lesions (60% vs 18.5%, P<0.01). MRP staining showed statistically significant correlations with shorter survival time and five-year survival rate (20.9±20.7 vs 48.5±22.7 months, 10% vs 65.6%, P<0.01). The results suggest that MRP expression is common in the human gastric carcinomas, and might be related to the intrinsic drug resistance. Immunohistochemical detection of MRP might be useful in selecting chemotherapy and predicting prognosis.
出处
《上海第二医科大学学报》
CSCD
1997年第4期247-250,共4页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
耐药相关蛋白
胃癌
免疫组化
预后
multidrug resistance-associated protein
gastric neoplasms
immunohistochemistry
prognosis
