双歧杆菌在短肽肠内营养液中的存活力

Viability of bifidobacteria in short peptide enteral nutrition solution

目的:研究双岐杆菌在短肽肠内营养液中的存活力.方法:将50g/L两歧双歧杆菌菌悬液加入120g/L短肽营养液中,37℃厌氧培养箱中培养,分别在0,4,8,12,14,16,20,24h时间点取样进行活菌数、A550、酸度和pH测定.结果:在短肽肠内营养液中接种两歧双歧杆菌后,活菌数在培养0～16h生长速度较快,16h达最高峰,以后下降.A550随着时间的延长而增加.酸度也随着培养时间的延长而增加,在培养0～14h之间增加较快,14h以后增加缓慢.pH值随培养时间的延长而下降,在培养0～12h之间下降较快,12h以后下降缓慢.结论:双歧杆菌能在120g/L的短肽肠内营养液中较好地存活,且在12～14h期间应停止发酵.

AIM： To investigate the viability of bifidobacteria in short peptide enteral nutrition solution. METHODS： 50 g/L fermentative medium of bifidobacteria was added in 120 g/L short peptide enteral nutrition solution. The preparations were cultured at 37℃ anaerobic condition. Samples were taken at 0, 4, 8, 12, 14, 16, 20, 24 h during fermentation. Bacteria counts, A550, acidity and pH values were examined respectively. RESULTS： The number of bifidobacteria increased at 0-16 h, peaked at 16 h and declined after 16 h. A550 ascended at all time. Acidity significantly increased at 0-14 h, and slowly after 14 h. PH values significantly decreased at 0-12 h, and slowly after 12 h. CONCLUSION： Bifidobacteria have better viability in 120 g/L short peptide enteral nutrition solution, and should cease ferment during 12-14 h.