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胆碱受体激动剂对谷氨酸诱导小胶质细胞增殖的影响 被引量：1

Effects of cholinergic agonists on microglial proliferation induced by glutamate
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摘要 目的:观察尼古丁及卡巴胆碱对谷氨酸诱导的小胶质细胞增殖的影响.方法:培养的小胶质细胞系,加入不同浓度的尼古丁、卡巴胆碱及谷氨酸,用MTT方法检测细胞活性,观察对小胶质细胞数量的变化.结果:培养的小胶质细胞提示,尼古丁及卡巴胆碱显著减少谷氨酸诱导小胶质细胞数量的增多,有剂量依赖效应,10μmol/L浓度效果最为显著.结论:尼古丁及卡巴胆碱可显著抑制谷氨酸诱导小胶质细胞活化的增殖. AIM： To observe the effects of nicotine and carbachol on the proliferation of microglia induced by glutamate. METHODS： The number of cultured microglia treated with different doses of nicotine, carbachol and glutamate respectively was determined by MTT assay. RESULTS： Nicotine and carbachol decreased the number of microglia in the presence of glutamate in a dose-dependent manner, and most significantly at 10 μmol/L concentration of nicotine or carbachol. CONCLUSION ： Nicotine and carbachol inhibit significantly microglial proliferation induced by glutamate.
作者 关艳中 金秀东 刘桂莲 高天明
机构地区 南方医科大学神经生物学教研室 牡丹江医学院生理学教研室
出处 《第四军医大学学报》 北大核心 2007年第22期2041-2043,共3页 Journal of the Fourth Military Medical University
基金 国家自然科学基金(30330240 U0632007)
关键词 胆碱能激动剂 谷氨酸 小神经胶质细胞 细胞增殖 cholinergic agonists glutamic acid microglia cell proliferation
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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同被引文献10

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  • 10关艳中,高天明.卡巴胆碱对参与中枢炎症反应的小胶质细胞增殖的影响[J].中国临床解剖学杂志,2007,25(6):677-680. 被引量：1

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第四军医大学学报
2007年 第22期

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