强直性脊柱炎与HLA-B2704,05亚型分布关系

Distribution of HLA-B2704,05 subtypes in patients with ankylosing spondylitis

目的:用流式芯片以及PCR技术检测HLA-B2704,05亚型,提供临床诊断强直性脊柱炎的新方法.方法:对124例强直性脊柱炎患者及50例正常人采用流式细胞方法检测HLA-B27,采用流式芯片方法检测HLA-B27亚型.结果:124例强直性脊柱炎患者HLA-B27阳性数116例,阳性率94%,其中HLA-B2704亚型68例,占总阳性数的58.6%,HLA-B2705亚型48例,占总阳性数的41.4%.50例正常人的亚型检测结果均为HLA-B2704阳性.结论:强直性脊柱炎患者的发病机制与各亚型的特异序列无关,仅与HLA-B27各亚型共有序例有关,流式芯片技术可以快速准确地对HLA-B27相关亚型作出分析,为深入探讨强直性脊柱炎发病机制奠定工作基础,并提供强直性脊柱炎临床诊断的新方法.

AIM： To evaluate the subtypes of homologous leucocytic antigen B27 （HLA-B27） with the fluidic chip assay and PCR technique so as to provide a new method for diagnosing ankylosing spondylitis （AS）. METHODS： Flow cytometry and PCR-fluidic chip technique were respectively used to detect HLA-B27 and assay the subtypes of HLA-B2704 and HLA-B2705 in 124 patients with AS and 50 health adults. RESULTS： 94% of the 124 patients with AS （116/124） were HLA-B27 positive. Among them 68 samples were HLA-B2704 positive, accounting for 58.6% （68/116） and 48 samples were HLA-B2705 positive with a percentage of 41.4% （48/116）. Only one HLA-B2704 positive specimen was found in all the 50 healthy samples. CONCLUSION： The pathogenesis of AS might be independent with the specific sequences of the subtypes in HLA-B27 and only related to the consensus sequence of the subtypes. The fluidic chip technique can make an accurate and fast analysis on HLA-B27 relevant subtypes. This study establishes a foundation for probing into the pathogenesis of AS and offers a new clinical diagnosis method of AS.