健康成人肝脏^(31)P-MR波谱研究

Hepatic ^(31)P-MR Spectroscopy Analysis of Normal Adults

目的:探讨健康成人肝脏31P-MR波谱及代谢生化信息特点。方法:分析20例健康志愿者31P-MR波谱检查肝脏代谢化合物信息及细胞内pH值,并将本组研究对象按年龄分为3组,分别为青年组(20～34岁)、中年组(35～49岁)和老年组(50～65岁),统计分析各年龄组间肝脏代谢物水平是否有差异。结果:20例研究对象肝脏31P-MRS均能显示磷酸单脂(PME)、无机磷(Pi)、磷酸双脂(PDE)、磷酸肌酸(PCr)、γ-ATP、α-ATP和β-ATP共7种代谢产物峰(从左到右),其波峰化学位移分别为0.7～1.9、0.44～-0.75、-1.45～-2.65、-4.81～-6.13、-7.3～-8.46、-12.7～-14.05和-21.45～-22.77ppm,其峰下积分面积分别为0.8±0.57、0.97±0.68、2.51±1.59、0.61±0.5、2.11±0.99和1.14±0.85,计算肝组织PH值为7.35±0.04。经方差分析,各年龄组间PME、Pi、PDE、γ-ATP、α-ATP和β-ATP代谢物峰下面积和细胞内PH值均无统计学差异(P>0.05),肝脏代谢化合物含量、能量状况及细胞内PH值不随年龄改变而变化。结论:肝脏31P-MRS能无创、直接检测肝代谢过程的生化信息及能量状况,对了解肝脏代谢情况具有重要临床价值。

Objective： To study the features of hepatic metabolic information of normal adults with^31 P-MR spectroscopy in vivo. Methods：20 healthy adults were selected and were divided into 3 groups, designated as youth group （20-34 years of age） ,middle aged group （35-49 years of age） and elderly group （55-65 years of age）. Hepatic ^31 P-MRS was performed in all the adults in these 3 groups and hepatic metabolic compound information and the intracellular pH values of each aged group were respectively studied and statisticrtlly analyzed. Results： 6 metabolite peaks, namely PME, Pi, PDE, γ- ATP,α-ATP and β-ATP were all demonstrated on the hepatic ^31 P-MRS images in the adults of all groups. The chemical shifts of the peaks of these metabolic compounds were 0.7-1.9,0.44-0.75,-1.45- -2.65,-4.81- -6.13,-7.3- -8.46,- 12.7- 14.05 and -21.45-22.77ppm respectively,and the integrated areas beneath the relative peaks were 0. 8±0. 57,0. 97±0. 68,2. 51±1. 59,0, 61±0.5,2. ll±0.99 and 1.14±0.85 respectively. The pH of liver was measured as 7.35 ±0.04. Statistically, the differences of integrated areas beneath the relative peaks and the intracellular pH in the 3 different aged groups revealed no remarkable significance （P〉 0.05）. Conclusion： Hepatic ^31 P-MRS might non-invasively provided the metabolic biochemical information and the energy status of the liver, clinically,it was a valuable imaging examination modality for the understanding of the situation of liver metabolism.