早期血清蛋白S-100B变化与新生儿窒息的预后关系分析

Study on the correlation between serum S-100B protein and neonatal asphyxia

目的通过早期检测血清S-100B在新生儿窒息中的水平的动态变化,探讨其对脑损伤的早期发现及远期(12月时)预后判断的临床价值。方法采用酶联免疫吸附试验双抗体夹心法测定102例有窒息史的新生儿出生～24h、出生后48～72h和生后7d的血清S-100B水平,同时随访至12月并检测发育上(DQ),与正常对照组30名健康新生儿进行回顾性比较分析。结果窒息儿中各时间段血清S-100B蛋白水平,均高于正常对照组(P<0.01),各组中HIE病儿的S-100B蛋白水平明显高于无HIE症状者。出生～24h、出生后48～72h血清S-100B蛋白水平升高者,12月时DQ明显异常。结论血清S-100B蛋白可作为早期发现窒息儿中是否存在脑损伤(特别是HIE)的主要指标及判断其远期预后的客观依据之一。

Objective：To study clinical significance of the serum level of S-100B protein in the asphyxial neonates,brain damage , i.e, hypexie-ischemie eneephalopathy（HIE）ean be found in early time.To treatment in the early can reduce the occurrence of the neurological complications .Methods： Thirty eases of healthy infants were regarded as control group （group 1）. One-hundred and two eases of asphyxial neonates were divided into two groups （40 eases without HIE as group 2; another 62 eases with HIE as group 3）. S-100B protein levels in serum of three groups were measured with enzyme-linked immunosorbent assay retrospectively. Results： The first measument,levels of S-100B protein in group 1, group 2 and group 3 were （0.08±0.02）μg/L, （1.86±1.13）μg/L and （2.76±1.96）μg/L, respectively. S-100B protein levels in the serum of group 2 and group 3 were significantly higher than that of group 1. S-100B protein level in the serum of group 3 was significantly higher than that of group 2. Condusion： S-100B protein level in the serum may be helpful in the early diagnosis of asphyxial neonates with brain damages espiealy HIE.In the first week after perinatal asphyxiation,measuring S-100B is useful to predict the outcome in the long-term.