人参单体皂甙Rh_2对非小细胞肺癌患者肺泡巨噬细胞免疫活性的影响

Effect of ginsenoside Rh_2 on immunocompetence of alveolar macrophages in patients with non-small cell lung cancer

目的:探讨人参单体皂甙Rh2(G-Rh2)对非小细胞肺癌患者肺泡巨噬细胞(AM)分泌细胞毒效应分子的影响。方法:采用ELISA法和酶法分别检测35例非小细胞肺癌患者支气管肺泡灌洗液及其AM培养上清液中肿瘤坏死因子(TNF-α)和NO浓度,并分别用干扰素-α(IFN-α)、G-Rh2以及两药联合干预AM培养,检测其上清液中TNF-α和NO变化情况。结果:所有非小细胞肺癌患者其AM均可产生一定量的TNF-α和NO;非小细胞肺癌患者荷瘤侧肺NO和TNF-α活性在肺泡灌洗液及AM培养上清液中均明显低于非荷瘤侧肺。G-Rh2干预下AM培养上清液中TNF-α和NO的浓度较对照组明显升高(P<0.05);与TNF-α干预AM培养上清液中TNF-α和NO的浓度比较差异无统计学意义(P>0.05)。G-Rh2和TNF-α联合干预时AM培养上清液中TNF-α和NO的浓度明显大于TNF-α或G-Rh2单独干预(P<0.01)。结论:G-Rh2能促进非小细胞肺癌患者肺泡巨噬细胞分泌细胞毒效应分子,联合干扰素时其作用更显著,两者具有协同性。

Objective To explore the effect of ginsenoside Rh2 （G-Rh2 ） on the excretion of cytotoxin- effecting moleule of alveolar macrophages （ AM ） in patients with non-small cell lung cancer （NSCLC）. Methods The concentration of tumor necrosis factor （TNF-α） and NO in the bron- choalveolar lavage fluid （BALF） and the cultured supernatants of AM in 3 5 patients with NSCLC were measured by ELISA and enzyme method, and levels of TNF-α and NO in the cultured supernatants of AM after being cultivated with IFN-α, G-Rh2, and IFN-α ＋ G-Rh2 were measured by the same method. Results AM in all the non-small cell lung cancer patients produced TNF-α and NO. The activity of TNF-α and NO was lower in the BALF and in the cultured supernatants of AM of the tumor-bearing lungs than that of the non-tumor-bearing lungs. The concentrations of TNF-α and NO in the cultured supernatants of AM cultivated with G-Rh2 were higher than those in the control （P 〈 0.05 ） , but there were no significant differences between the G-Rh2 group and IFN-α group （P 〉 0.05）. The concentrations of TNF-α and NO in the cultured supernatants of AM cultivated with both G-Rh2 and IFNet were obviously higher than those stimulated with IFNα or G-Rh2 （ P 〈 0. 01 ） alone. Conclusion G-Rh2 can enhance the excretion of cytotoxin-effecting moleules of AM in pa-tients with NSCLC. The changes are more distinctive when G-Rh2 and IFNα have coordinated action.