槲皮素对小鼠移植宫颈癌的抑制作用

Inhibitory effect of quercetin on cervix cancer in mice

目的:探讨槲皮素对小鼠宫颈癌U14的抗癌作用及其可能机制。方法:建立615近交系小鼠U14移植瘤动物模型,并随机分成4组:对照组、低剂量干预组[1.5g/(kg·d)]、中剂量干预组[3.0g/(kg·d)]及高剂量干预组[6.0g/(kg·d)],腹腔注射给药,连续用药20d,于接种后26d处死各组小鼠,检测各组小鼠的瘤体平均质量并计算抑瘤率,采用免疫组织化学半定量检测肿瘤组织微血管密度(MVD)及核因子-κB(NF-κB)表达水平,用原位TdT检测法(TUNEL)检测肿瘤细胞凋亡指数(AI)。结果:高剂量干预组肿瘤的生长明显受到抑制,肿瘤体积和瘤质量均明显低于对照组(P<0.01),其抑瘤率显著高于低、中剂量干预组(P<0.01);高剂量干预组MVD及NF-κB表达水平明显降低,AI则明显升高,与对照组比较,差异均有统计学意义(P<0.01),但低、中剂量槲皮素对MVD,NF-κB表达及AI无明显影响(P>0.05)。结论:槲皮素对小鼠宫颈癌的生长具有显著的抑制作用,其抗癌机制可能与抑制微血管生成和促进细胞凋亡有关。

Objective To investigate the antitumor effect and mechanism of quercetin on mufine cervical carcinomal U14. Methods The 615-strain mice with U14 cervical cancer cells were randomly divided into 4 groups： a control, a low-dose intervention group [ 1.5 g/（kg·d） ] , a mid- dle-dose intervention group [ 3.0 g/（ kg·d） ] , and a high-dose intervention group [6.0 g/（ kg·d ） ] . Different treatments were inoculated intraperitoneally after 6 days of transplantation and all mice were sacrificed after 26 days. The weight of tumors and inhibitory rates were measured. The expression levels of microvessel density （ MVD ） and nuclear factor- KappaB （ NF-κB ） were detected by immunhistochemistry. The apoptosis index （AI） was measured by terminal deoxynucleotydyl transferase assay in situ （TUNEL）. Results Compared with the control group, the tumor growth in the high-dose intervention group was suppressed significantly, and the weight and volume of the tumor were markedly decreased （P 〈0.01 ）. The inhibitory rate in the high-dose intervention group was higher than that in the low- and middle-dose groups （ P 〈 0. 05 ） . The expression levels of NF-κB and MVD were significantly decreased, and AI was enhanced in the high-dose intervention group （P 〈0.01）. However, the low- and middle-dose quercetin had no obvious influence on the NF-κB ex-pression, MVD and AI （ P 〉 0. 05 ）. Conclusion Quercetin showed a marked inhibitive effect on U14 growth, and its antitumor mechanism may be associated with inhibiting the angiogenesis and inducing apoptosis.