摘要
将次氯酸钠用于微生物胞内产物聚β－羟丁酸酯（ＰＨＢ）的提取中。发现在ｐＨ９．８，菌体干重／次氯酸钠（ｗ／ｖ）１％的条件下作用１ｈ得到的ＰＨＢ纯度达９５％，粘均分子量Ｍη为３．２×１０５。进一步在次氯酸钠使用前用十二烷基硫酸钠（ＳＤＳ）破胞，得到的ＰＨＢ在同样的纯度下，Ｍη增至５．２×１０５．
Hypochlorite digestion was applied in the extraction of intracellular poly β hydrobutyrate(PHB).The optimum treatment conditions were found to be pH 9.8,Biomass/hypochlorite (w/v) 1% and treating time 1 hr.PHB with 95% purity and average molecuiar weight (Mη) 3.2×10 5 were obtained.When the cells were treated with surfactant sodium sulfate (SDS) before digestion,the molecular weight (Mη) of PHB increased to 5.2×10 5 with the same purity.
基金
国家自然科学基金
