摘要
本研究对有色家兔视网膜色素上皮（ＲＰＥ）细胞进行体外培养，结果显示：低浓度上皮生长因子ＥＧＦ对ＲＰＥ细胞无刺激作用，而达到适当浓度时对ＲＰＥ细胞有明显刺激作用，但高浓度与适当浓度的作用相同。本文认为适当浓度的ＥＧＦ可促进ＲＰＥ培养细胞生长，缩短传代周期，细胞增殖速度快，细胞数量明显增多，且细胞形态不受影响。ＥＧＦ对体外培养ＲＰＥ细胞的最佳浓度为１０ｎｇ／ｍｌ。
The Effects of EGF on Cultured rabbit retinal pigment epithelial cells in vitro were studied.The results showed that low concentration EGF had no stimulatory effects on RPE cells,but proper concentration had significantly stimulatory effects,and the effect of high concentration were equal to that of proper concentration.This study suggested that proper concentration EGF could accelerate RPE cell growth,promote the cell proliferation,increase the cell amount,and not affect the shape of cells.The best concentration of EGF in RPE cell culture was 10ug/ml,this provided a new laboratory data for RPE cell culture in vitro.
出处
《中国实用眼科杂志》
CSCD
1997年第6期343-345,共3页
Chinese Journal of Practical Ophthalmology
基金
卫生部科学基金
