纳米羟基磷灰石颗粒与成骨细胞相容性的研究

Experimental Research on the Effect of Nano-Hydroxyapatite on Osteoblasts Functions

目的:将纳米羟基磷灰石颗粒(nano-hydroxyapatite,nHA)与体外培养的胎鼠颅顶骨源性成骨细胞共同培养,观察nHA与成骨细胞的相容情况及细胞形态。评价其细胞增殖性和细胞相容性。方法:将体外培养的胎鼠颅顶骨源性成骨细胞培养于标准培养基中,每个培养基中添加不同浓度的nHA颗粒,37.5℃、5%CO2孵箱内培养120 h。并以两个无添加物的培养基作为空白对照。分别在2、6、8、24、72、120 h测定成骨细胞的数量,并用透射电镜和倒置相差显微镜分别观察成骨细胞的形态和细胞显微结构。结果:实验1、2、3组在24、72、120 h时MTT吸光值与对照组比较差异均有统计学意义(P均<0.05)。实验1、2、3组在2、6、8、24、72、120 h时成骨细胞数目与对照组比较差异均有统计学意义(P均<0.05)。透射电镜观察结果显示,成骨细胞的细胞突中吸收了纳米颗粒。倒置相差显微镜观察结果显示,传代细胞在6 h内贴壁,细胞形态为长梭形、鳞片形、短梭形、圆形。结论:nHA材料具有很好的安全性和细胞相容性。

Objective： To investigate the osteoblast＇s biocompatibility and shape in vitro. Methods： The osteoblasts from rat calvaria were cultured with different containing of nano-hydroxyapatite （10,100,500 mg/L） vitros and two conventional vitros in 37.5℃, 5 % CO2 box. Osteoblast were observed under light microscope and electronic scanning microscope and measured the number of osteoblasts at 2,6,8,24,72 and 120 hours. Results：In groups 1,2,and 3 the MTT,compared with that in contrast group at 24,72 and 120 h,showed a statistic differences （P〈0.05） ;the number of the osteoblasts in Groups 1,2, and 3, compared with that in contrast group at 2,6,8,24,72 and 120 h, showed a statistic differences （P〈0.05） ;under the light microscope and electronic scanning microscope,the osteoblast＇s process had absorbed the nano-hydroxyapatite and the osteoblast sticked to the side within 6 hours meanwhile the osteoblast＇s shape is the long shuttle, scale, short shuttle,round. Conclusion：The nano-hydroxyapatite possesses excellent safety and biocompatibility.